CD56 Antibody 710388 from Thermo Fisher Scientific

Supplier Images

WB

Western blot was performed using Anti-CD56 Recombinant Polyclonal Antibody (3HCLC) (Product # 710388) and bands in the range of 130-240 kDa corresponding to Neural cell adhesion molecule 1 were observed. Membrane enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), IMR-32 (Lane 2), NK-92 (Lane 3), SK-N-SH (Lane 4), MCF7 (Lane 5), SK-BR-3 (Lane 6) and T-47D (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20,000 using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). Relative expression was observed between SH-SY5Y, IMR-32, NK-92, SK-N-SH and breast cancer cell lines such as MCF7, SK-BR-3 and T-47D as expected (DOI: 10.1038/s41598-019-45377-8).
ICC/IF

Immunofluorescence analysis of Neural cell adhesion molecule 1 was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD56 Recombinant Polyclonal Antibody (3HCLC) (Product # 710388) at 1 ug/ml in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Plasma membrane localization. Panel e represents the merged image of MCF7 cells showing very low expression of CD56 as expected (DOI: 10.1038/s41598-019-45377-8). Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
CD56 Antibody

Citations:

Supplier Page from
Thermo Fisher Scientific for
CD56 Antibody

Thermo Fisher Scientific's CD56 Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Flow Cytometry, Immunocytochemistry, and Western Blot. The CD56 Antibody was generated using NCAM1 as the antigen and it reacts with Homo Sapiens, Human, and Rat.

WB

ICC/IF

CD56 Antibody

CD56 Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

Citations (2)

Reviews

CD56 Antibody from Thermo Fisher Scientific

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map